Formalin-fixed and paraffin-embedded human spleen tissue reacted with BAT4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of BAT4 (arrow) using rabbit polyclonal BAT4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BAT4 gene.
Western blot of BAT4 Antibody in mouse spleen tissue lysates (35 ug/lane). BAT4 (arrow) was detected using the purified antibody.
BAT4 Antibody flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.