Western blot analysis of extracts from various cell lines using INTS9 Antibody.
Immunoprecipitation of INTS9 from HCT 116 cell extracts using Normal Rabbit IgG #2729 (lane 2) or INTS9 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using INTS9 Antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 10 µl of INTS9 Antibody or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human RNU2-1 Promoter Primers #14084 and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.