Puma (D7L9L) Rabbit mAb (Rodent Specific)

• Supplier: Cell Signaling Technology
• Catalog: 24633
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D7L9L
• Applications: WB IP ICC/IF
• Species Reactivities: Mouse, Rat
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg84 of mouse Puma protein.
• Description: Rabbit Monoclonal
• Gene: Bbc3
• Conjugate: Rodent Specific

Product images

Western blot analysis of extracts various cell lines using Puma (D7L9L) Rabbit mAb (Rodent Specific) (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length mouse Puma (mPuma; +) using Puma (D7L9L) Rabbit mAb (Rodent Specific).

Immunoprecipitation of Puma from BaF3 cell extracts. Lane 1 represents 10% input, lane 2 is immuoprecipitated with Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is Puma (D7L9L) Rabbit mAb (Rodent Specific). Western blot was performed using Puma (D7L9L) Rabbit mAb (Rodent Specific). A conformation specific secondary antibody was used to avoid cross reactivity with IgG.

Confocal immunofluorescent analysis of BaF3 (high expressing; left) and J744A.1 (low expressing; right) using Puma (D7L9L) Rabbit mAb (green; upper). Mitochondria were co-labeled with COX IV (4D11-B3-E8) Mouse mAb #11967 (red; lower). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).