Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent confocal image of HeLa cell stained with CNOT8 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.
CNOT8 Antibody western blot of U251 cell line lysates (35 ug/lane). The CNOT8 antibody detected the CNOT8 protein (arrow).
Flow cytometric of CEM cells using CNOT8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.