Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or Jak2 siRNA (+), using Jak2 (D2E12) XP ® Rabbit mAb #3230 and α-Tubulin (11H10) Rabbit mAb #2125. The Jak2 (D2E12) XP ® Rabbit mAb confirms silencing of Jak2 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Jak2 siRNA.
Western blot analysis of extracts from K-562, THP-1, TF-1 and BaF3 cell lines using Jak2 (D2E12) XP ® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP ® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP ® Rabbit mA in the presence of control peptide (left) or Jak2 Blocking Peptide #1039 (right).
Confocal immunofluorescent analysis of K-562 cells using Jak2 (D2E12) XP ® Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Flow cytometric analysis of K-562 cells using Jak2 (D2E12) XP ® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).