Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for XXXXX
Confocal immunofluorescent of HIF1Alpha Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot of HIF1Alpha Antibody in MDA-MB231 cell line lysates (35 ug/lane). HIF1Alpha (arrow) was detected using the purified antibody.
Western blot of HIF1Alpha Antibody in mouse cerebellum tissue lysates (35 ug/lane). HIF1Alpha (arrow) was detected using the purified antibody.