HIF1Alpha Antibody IHC of formalin-fixed and paraffin-embedded lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the HIF1Alpha Antibody for immunohistochemistry.
Confocal immunofluorescent of HIF1Alpha Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Western blot of HIF1Alpha Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane). HIF1Alpha (arrow) was detected using the purified antibody.
HIF1Alpha Antibody flow cytometry of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.