Anti-HIF1A / HIF1 Alpha Antibody (aa1-30)
| Name | Anti-HIF1A / HIF1 Alpha Antibody (aa1-30) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C166866 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P IHC-F WB IP FC ELISA ChIP |
| Species Reactivities | Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate |
| Purity/Format | Ammonium sulfate precipitation |
| Blocking Peptide | HIC2 Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | HIF1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
HIF1A Antibody IHC of formalin-fixed and paraffin-embedded lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Fluorescent confocal image of HeLa cell stained with HIF1A Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIF1A primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HIF1A immunoreactivity is localized to cytoplasm and nucleus significantly.
Western blot of HIF1A Antibody in CHO cell line lysates (35 ug/lane). HIF1A (arrow) was detected using the purified antibody.