Phospho-c-Jun (Ser73) (D47G9) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 3270
• Prices: $119.00, $318.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D47G9
• Applications: WB IP IHC-P ICC/IF FC ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Pig, Bovine
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser73 of human c-Jun.
• Description: Rabbit Monoclonal
• Gene: JUN

Product images

Western blot analysis of extracts from NIH/3T3 or C6 cells, untreated or UV-treated, using Phospho-c-Jun (Ser73) (D47G9) XP ® Rabbit mAb (upper) or c-Jun (60A8) Rabbit mAb #9165 (lower).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda phosphatase-treated (right), using Phospho-c-Jun (Ser73) (D47G9) XP ® Rabbit mAb.

Immunohistochemical analysis of parafin-embedded human colon carcinoma using Phospho-c-Jun (Ser73) (D47G9) XP ® Rabbit mAb in the presence of control peptide (left) or Phospho-c-Jun (Ser73) Blocking Peptide (right).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-c-Jun (Ser73) (D47G9) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using Phospho-c-Jun (Ser73) (D47G9) XP ® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Flow cytometric analysis of HeLa cells, untreated (blue) or UV treated (green), using Phospho-c-Jun (Ser73) (D47G9) XP ® Rabbit mAb.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 PC-12 cells starved overnight and treated with Human β-Nerve Growth Factor (hβ-NGF) #5221 (50 ng/ml) for 2h and either 10 μl of Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR SimpleChIP ® using Rat CCRN4L Promoter Primers #7983, rat DCLK1 promoter primers, and SimpleChIP ® Rat GAPDH Promoter Primers #7964. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.