Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of lysate from HeLa cell line, using Phospho-H3-pS10 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of extracts from HeLa cells,untreated or treated with FBS and Calyculin A,100nM, using phospho-H3-Ps10(left) or H3 antibody(right)
Western blot of extracts from HeLa cells,untreated or treated with FBS and Calyculin A ,100nM, using phospho-HIST1H3B3-S10(left) or HIST1H3B3 antibody(right)
Dot blot of anti-Phospho-HIST1H3B3-S10 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.