Lane 1: HT29 Cell lysates; Lane 2: mouse Cerebrum Cell lysates; Lane 3: Mouse kidney lysates; Lane 4: Mouse pancreas lysates; probed with DNAJC21 Polyclonal Antibody, unconjugated (bs-14387R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded mouse placenta tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DNAJC21 Polyclonal Antibody, Unconjugated (bs-14387R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.