Western Blot analysis of HOXD8 expression in transfected 293T cell line by HOXD8 monoclonal antibody (M01), clone 10F8.Lane 1: HOXD8 transfected lysate(31.8 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged HOXD8 is approximately 0.03 ng/ml as a capture antibody.
Western blot analysis of HOXD8 over-expressed 293 cell line, cotransfected with HOXD8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HOXD8 monoclonal antibody (M01), clone 10F8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.