HeLa cells staining with HSBP1 antibody (red), and counterstained with chicken polyclonal antibody to Vimentin (green) and DNA (blue). The HSBP1 antibody antibody reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.
Western blots of HeLa cell crude extracts. Lane 16 was probed with HSBP1 antibody, while lanes 14 and 15 were probed with two other monoclonals to HSP27 we generated at the same time. Note the strong clean bands at 27 kDa. Lane 17 was probed with MCA-4C4, our new mouse monoclonal antibody to Lamia A/C, which binds two bands running at 74 kDa and 65 kDa. Lane 13 was probed with MCA-5J11, our monoclonal antibody to all six actin isotypes. Molecular weights of each protein are as indicated, and dots indicate the presence of major HeLa proteins.