Formalin-fixed and paraffin-embedded human brain tissue reacted with HSPA8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent of HSPA8 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot of lysates from A431,HeLa,mouse NIH/3T3,H-4-II-E cell line (from left to right),using HSPA8 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
HSPA8 Antibody flow cytometry of HeLa cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.