Phospho-HSP27 (Ser82) (D1H2F6) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate)
| Name | Phospho-HSP27 (Ser82) (D1H2F6) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 12172 |
| Prices | $329.00 |
| Sizes | 100 µl (50 tests) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | D1H2F6 |
| Applications | ICC/IF FC |
| Species Reactivities | Human, Mouse, Rat, Hamster, Bovine, Dog, Horse |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser82 of human HSP27 protein. |
| Description | Rabbit Monoclonal |
| Gene | HSPB1 |
| Conjugate | Alexa Fluor ® 488 |
| Supplier Page | Shop |
Product images
Confocal immunofluorescent analysis of HeLa cells, untreated (top left) or UV-treated (top right), C2C12 cells, untreated (bottom left) or treated with λ phosphatase (bottom middle), and NIH/3T3 cells (bottom right) using Phospho-HSP27 (Ser82) (D1H2F6) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Negative staining in NIH/3T3 cells is in agreement with the observation that NIH/3T3 cells do not express HSP27 under basal conditions (5,7).
Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-HSP27 (Ser82) (D1H2F6) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate).