LC3A/B (D3U4C) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12741
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D3U4C
• Applications: WB IHC-P ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Xenopus, Bovine, Dog, Pig
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu44 of human LC3B protein (conserved in LC3A).
• Description: Rabbit Monoclonal
• Gene: MAP1LC3A

Product images

Western blot analysis of extracts from RD cells, untreated (-) or Torin 1-treated (250 nM, 4 hr; +), using LC3A/B (D3U4C) XP ® Rabbit mAb.

Western blot analysis of extracts from HeLa, NIH/3T3, and KNRK cells, untreated (-) or chloroquine-treated (50 μM, overnight; +), using LC3A/B (D3U4C) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse prostate using LC3A/B (D3U4C) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded NIH/3T3 cell pellets, control (left) or chloroquine-treated (right), using LC3A/B (D3U4C) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using LC3A/B (D3U4C) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Confocal immunofluorescent analysis of HeLa (upper) and C2C12 (lower) cells, chloroquine-treated (50 μM, overnight; left), nutrient-starved with EBSS (3 hr, middle) or untreated (right) using LC3A/B (D3U4C) XP ® Rabbit mAb (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor ® 555 Conjugate) #8046 (red). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of HeLa cells, untreated (blue) or treated with chloroquine (50 µM, 16 hr) (green), using LC3A/B (D3U4C) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) #4414 was used as a secondary antibody.