Confocal immunofluorescent analysis of NK-92 cells, treated with TPA #4174 and Ionomycin #9995 (80 nM and 3 uM, 4 hr; left) or untreated (right), using IFN-γ (D3H2) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of human peripheral blood cells, untreated (left) or treated with TPA #4174 (40 nM, 4 hr), Ionomycin, Calcium Salt #9995 (2 µM, 4 hr), and Brefeldin A #9972 (1 µg/ml, last 3 hr of stimulation) (right), using a CD3 antibody and IFN-γ (D3H2) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate). Analysis was performed on gated lymphocyte cells.