Anti-Mouse IL-27/p28 Antibody - Western Blot. Detection of recombinant IL27/p28 protein by anti-Mouse IL-27/p28 antibody. Recombinant mouse IL27/p28 was loaded on to an SDS-PAGE gel at 0.25 ug and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40000 in blocking buffer (MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.