Detection of Human INF2 by Western Blot and Immunoprecipitation. Samples : Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-INF2 antibody A303-42 9A used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. INF2 was also immunoprecipitated by rabbit anti-INF2 antibodies A303-427 A and A303-42 8 A, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).