Anti-ING3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B385 concentration 5 ug/ml.
![Anti-ING3 Antibody - Western Blot. Western blot of purified anti-ING3 antibody shows detection of a band at ~55 kD corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kD and 20 kD has not been determined. Each lane contains approximately 10 ug of RKO whole cell lysate (ATCC# CRL-2577 - human colon cancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membrane was probed with the primary antibody diluted to 1:1000. Incubation was at 4C overnight followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://www.bioprodhub.com/system/product_images/ab_products/3/sub_3/15060_62761_49227.jpg)
Anti-ING3 Antibody - Western Blot. Western blot of purified anti-ING3 antibody shows detection of a band at ~55 kD corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kD and 20 kD has not been determined. Each lane contains approximately 10 ug of RKO whole cell lysate (ATCC# CRL-2577 - human colon cancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membrane was probed with the primary antibody diluted to 1:1000. Incubation was at 4C overnight followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.