Western blot analysis of extracts from COS-7 and HeLa cells, untreated (-) or treated with Trichostatin A (TSA) #9950 (1 µM, 6 hr; +), using Acetylated-Lysine (Ac-K-100) MultiMab™ Rabbit mAb mix.
Western blot analysis of extracts from various mouse tissues using Acetylated-Lysine (Ac-K-100) MultiMab™ Rabbit mAb mix.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 10 μl of Acetylated-Lysine (Ac-K-100) MultiMab™ Rabbit mAb mix #9814 or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human RPL30 Exon 3 Primers #7014, SimpleChIP ® Human MYT-1 Exon 1 Primers #4493, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.