Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated as indicated, using Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (upper) or p38 MAPK Antibody #9212 (lower).
Confocal immunofluorescent analysis of HeLa cells either untreated (left) or anisomycin treated (right) labeled with Phospho-p38 MAP Kinase (Thr180/Tyr182) (28B10) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor Phalloidin 555 (red).
Flow cytometric analysis of Jurkat cells, untreated (green) or anisomycin-treated (blue), using Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb compared to a nonspecific negative control antibody (red).