Phospho-MARCKS (Ser167/170) (D13E4) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 8722
• Prices: $119.00, $318.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D13E4
• Applications: WB ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Chicken, Bovine
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser167 and Ser170 of human MARCKS protein.
• Description: Rabbit Monoclonal
• Gene: MARCKS

Product images

Western blot analysis of extracts from serum-starved SH-SY5Y and SK-N-MC cells, untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Phospho-MARCKS (Ser167/170) (D13E4) XP ® Rabbit mAb (upper) and MARCKS (D88D11) XP ® Rabbit mAb #5607 (lower).

Western blot analysis of extracts from various cell lines, serum-starved overnight and then untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Phospho-MARCKS (Ser167/Ser170) (D13E4) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of HeLa cells, serum-starved (left), treated with TPA #4174 (200 nM, 15 min; middle) or treated with TPA #4174 and λ phosphatase (right), using Phospho-MARCKS (Ser167/170) (D13E4) XP ® Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of HeLa cells, serum starved (blue) or serum starved and treated with TPA #4174 (200nM, 15 min; green), using Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary Ab.