Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells, serum starved overnight and either DMSO-treated or treated with TPA #4174 (200 nM, 15 min), using Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb.
Immunoprecipitation of phospho-MARCKS (Ser159/163) from HeLa cells, either DMSO-treated or treated with TPA #4174 (200 nM, 15 min), using Phospho-MARCKS (Ser159/163) (D13D2) Rabbit mAb (lanes 3 and 4) or MARCKS (D88D11) XP ® Rabbit mAb #5607 (lanes 5 and 6). Lanes 1 and 2 represent 10% of input lysate for each treatment. Western blot analysis was performed using MARCKS (D88D11) XP ® Rabbit mAb #5607. Note: MARCKS (D88D11) XP ® Rabbit mAb #5607 does not immunoprecipitate phospho-MARCKS (Ser159/163) and was used as a negative control.