Western Blot analysis of KCNJ15 expression in transfected 293T cell line by KCNJ15 monoclonal antibody (M01), clone 1B2.Lane 1: KCNJ15 transfected lysate(42.6 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged KCNJ15 is 3 ng/ml as a capture antibody.
Western blot analysis of KCNJ15 over-expressed 293 cell line, cotransfected with KCNJ15 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with KCNJ15 monoclonal antibody (M01), clone 1B2 . GAPDH ( 36.1 kDa ) used as specificity and loading control.