Western blot analysis of extracts from A431, COS and PC12 cells, untreated or nocodazole-treated, using Phospho-MEK1 (Thr286) Antibody (upper) or MEK1 Antibody #9124 (lower).
Immunoprecipitation followed by Western blot analysis of extracts from COS cells, untreated or nocodazole-treated, using Phospho-MEK1 (Thr286) Antibody.
Confocal immunofluorescent analysis of mitotic HeLa cells labeled with Phospho-MEK1 (Thr286) Antibody (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of untreated Jurkat cells, using Phospho-MEK1 (Thr286) Antibody versus propidium iodide (DNA content). The box indicates Phosho-MEK1 positive cells.