Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAGEA4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunostaining of paraformaldehyde-fixed and paraffin-embedded human breast carcinoma with MAGE-A4 antibody (1:50 dilution), followed by reacting with biotin-conjugated secondary antibody, ABC solution and developing with DAB. Cancerous cells show nuclear staining of MAGE-A4. Magnification: 40X. Data courtesy of Dr. Mi Hou, Karolinska?Institutet and University Hospital, Sweden.
The anti-MAGE-A4 antibody is used in Western blot to detect MAGE-A4 in A375 cell lysate.