Anti-MAP1LC3A / LC3A Antibody (aa91-117)
| Name | Anti-MAP1LC3A / LC3A Antibody (aa91-117) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C165696 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P WB |
| Species Reactivities | Human, Mouse, Rat, Bovine, Opossum, Zebrafish |
| Purity/Format | Protein A purified |
| Blocking Peptide | MAP1LC3A / LC3A Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | MAP1LC3A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Fluorescent image of U251 cells stained with LC3 (APG8A) (D106) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LC3 (APG8A) (D106) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Western blot of LC3 (APG8a) (arrow) using rabbit polyclonal Autophagy LC3 Antibody (APG8a) (D106). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the LC3 (APG8a) gene (Lane 2) (Origene Technologies).