Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-MARS mAb.
Western blot analysis of immunized recombinant protein, using anti-MARS monoclonal antibody.
Detection of MARS by Western blot. Samples: Whole cell lysate from human HEK293 (H, 25 ug) , mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. Predicted molecular weight: 101 kDa
Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-MARS mAb. Predicted molecular weight: 101 kDa