
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 K562 cells and either 10 µl of MTA1 (D17G10) XP ® Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human RPL30 Exon 3 Primers #7014, human BCR exon 1 primers, and SimpleChIP ® Human Nanog Promoter Primers #95064. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.