Western blot analysis of extracts from ZR-75-1, MCF 10A, and HCT 116 cells using MUC1 (D9O8K) XP ® Rabbit mAb (upper) and β-Tubulin Antibody #2146 (lower).
Confocal immunofluorescent analysis of ZR-75-1 (positive, left) and HCT 116 (negative, right) cells using MUC1 (D9O8K) XP ® Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of HCT 116 (blue) and ZR-75-1 (green) using MUC1 (D9O8K) XP ® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.