CXCL9/MIG in THP-1 Human Cell Line. CXCL9/MIG was detected in immersion fixed THP-1 human acute monocytic leukemia cell line stimulated with IFN-gamma using Human CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Chemotaxis Induced by CXCL9/MIG and Neutralization by Human CXCL9/MIG Antibody. Recombinant Human CXCL9/MIG chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL9/MIG (1 mg/ml) is neutralized (green line) by increasing concentrations of Human CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 3-12 ug/ml.