Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MSRA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescent of A549 cells, using MSRA Antibody. Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Western blot of MSRA antibody in mouse kidney tissue lysates (35 ug/lane). MSRA (arrow) was detected using the purified antibody.
MSRA Antibody flow cytometry of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.