Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 5482
• Prices: $119.00, $318.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D98G11
• Applications: WB IHC-P ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr346 of mouse NDRG1 protein.
• Description: Rabbit Monoclonal
• Gene: NDRG1

Product images

Western blot analysis of extracts from C2C12 cells, untreated or treated with LY294002 #9901, insulin, or both using Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb (upper) or total NDRG1 Antibody #5196 (lower).

Western blot analysis of extracts from A431 cells treated with hEGF #8916 (100 ng/ml, 30 minutes), with or without calf intestinal phosphatase and λ-phosphatase, using Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb (upper) or total NDRG1 Antibody #5196 (lower).

Western blot analysis of HeLa cells treated with hEGF #8916 (100 ng/ml, 30 minutes), mock transfected or transfected with SignalSilence ® NDRG1 siRNA I #6245 or SignalSilence ® NDRG1 siRNA II #6257, using Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower).

Immunohistochemical analysis on SignalSlide ® Phospho-Akt (Ser473) IHC Controls #8101 [paraffin-embedded LNCaP cell pellets, control (left) or LY294002-treated (right)] using NDRG1 Antibody #5196 (top) or Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb (bottom).

Immunohistochemical analysis of paraffin-emebedded mouse colon using Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-emebedded human lung carcinoma using Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of C2C12 cells, treated with LY294002 #9901 (left) or insulin (right), using Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, wortmannin #9951 and U0126 #9903 (blue), using Phospho-NDRG1 (Thr346) (D98G11) XP ® Rabbit mAb.