Anti-NANOG Antibody (Ser285)
| Name | Anti-NANOG Antibody (Ser285) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C98246 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P WB |
| Species Reactivities | Human, Monkey |
| Purity/Format | Protein A purified |
| Blocking Peptide | NANOG Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | NANOG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Fluorescent confocal image of SY5Y cells stained NANOG (S285) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated NANOG (S285) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
Western blot of NANOG (arrow) using rabbit NANOG Antibody (S285). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
Western blot of NANOG Antibody (S285) in HepG2 cell line lysates (35 ug/lane). NANOG (arrow) was detected using the purified antibody.