Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence of anti-NANOS1 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Western blot of anti-NANOS1 Antibody (RB13839) in T47D cell line lysates (35 ug/lane). NANOS1 (arrow) was detected using the purified antibody.
Flow cytometric of HeLa cells using Nanos Homologue 1 (NANOS1) Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.