BOLA2 Antibody

Name	BOLA2 Antibody
Supplier	Cusabio
Catalog	CSB-PA867143LA01HU
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Applications	ELISA IHC ICC/IF
Species Reactivities	Human
Antigen	Recombinant Human BolA-like protein 2 protein (1-86AA)
Purity/Format	>95%, Protein G purified
Description	Rabbit Polyclonal
Gene	BOLA2B
Conjugate	Non-conjugated
Supplier Page	Shop

Product images

<img /><p>IHC image of CSB-PA867143LA01HU diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.</p><p><a href="http://www.cusabio.cn/images/antibody/4/CSB-PA867143LA01HU-IHC-2.jpg" target="_blank"> </a></p>

IHC image of CSB-PA867143LA01HU diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

<img /><p>Immunofluorescence staining of Hela cells with CSB-PA867143LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).</p>

Immunofluorescence staining of Hela cells with CSB-PA867143LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).