NELL1 in Mouse Embryo. NELL1 was detected in immersion fixed frozen sections of mouse embryo (13 d. p. c.) using Sheep Anti-Mouse NELL1 Antigen Affinity-purified Polyclonal Antibody at 5 ug/ml overnight at 4°C. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons in the spinal cord.
Detection of Mouse NELL1 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Mouse NELL1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for NELL1 at approximately 110 kD (as indicated). This experiment was conducted under reducing conditions.