Anti-NFIA / Nuclear Factor 1 Antibody (aa470-498) IHC-plusâ¢
| Name | Anti-NFIA / Nuclear Factor 1 Antibody (aa470-498) IHC-plus⢠|
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-B9077 |
| Prices | $395.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P WB |
| Species Reactivities | Human, Monkey, Mouse, Rat, Bovine, Guinea Pig, Horse, Pig, Rabbit, Chicken, Xenopus |
| Purity/Format | Protein A purified |
| Blocking Peptide | NFI / NFIC Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | NFIA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-NFIA / Nuclear Factor 1 antibody IHC staining of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Fluorescent confocal image of HeLa cell stained with NFIA Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIA primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIA immunoreactivity is localized to Nucleus significantly.
NFIA Antibody western blot of HepG2 cell line lysates (35 ug/lane). The NFIA antibody detected the NFIA protein (arrow).