Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.This data demonstrates the use of Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody for immunohistochemistry.
Fluorescent confocal image of HeLa cells stained with Natriuretic Peptide Receptor C antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Natriuretic Peptide Receptor C primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Natriuretic Peptide Receptor C mainly to the mainly to the nucleus.
Western blot of ANPC-S82 in NCI-H460,HL-60 cell line lysates (35 ug/lane). ANPC (arrow) was detected using the purified antibody.