Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb
| Name | Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 4147 |
| Prices | $109.00, $287.00 |
| Sizes | 20 µl (2 western blots), 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D3B8 |
| Applications | WB IP ChIP |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence at the Val1754 cleavage site in human Notch1 (equivalent to Val1744 in mouse Notch1). |
| Description | Rabbit Monoclonal |
| Gene | NOTCH1 |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb (upper) or Notch1 (D1E11) XP ® Rabbit mAb #3608 (lower).
CUTLL1 cells were cultured in media with γ-secretase inhibitor (1μM) for 3 days and then either harvested immediately (left panel) or washed and cultured in fresh media for 3h (right panel). Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 cells and 2.5 µl of Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human HES1 promoter primers, SimpleChIP ® Human HES4 Promoter Primers #7273, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.