Western blot analysis of extracts of HeLa cells, untreated or nocodazole-treated, using Phospho-NPM (Thr95) Antibody (upper) or NPM Antibody #3542 (lower).
Confocal immunofluorescent analysis of HeLa cells untreated (left) and λ phosphatase-treated (right), using NPM (Thr95) phosphate Antibody (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Flow cytometric analysis of Jurkat cells using Phospho-NPM (Thr95) Antibody versus propidium iodide (DNA content). The box indicates phospho-NPM positive cells.