Detection of Human NUP93 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-NUP93 antibody used for IP at 6 ug/mg lysate. NUP93 was also immunoprecipitated by rabbit anti-NUP93 antibody BL14355. For blotting immunoprecipitated NUP93, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human NUP93 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-NUP93 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.