Western blot analysis of extracts from various cell lines using OB-Cadherin (16G5) Mouse mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). As expected, MCF7 cells are negative for OB-cadherin.
Confocal immunofluorescent analysis of H226 (positive, left) and MCF7 (negative, right) cells using OB-cadherin (16G5) Mouse mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of unpermeabilized LNCaP cells (blue) and unpermeabilized H460 cells (green) using OB-Cadherin (16G5) Mouse mAb. Anti-mouse IgG (H+L), F(ab')2 fragment (Alexa Fluor 488 conjugate) #4408 was used as a secondary antibody.