Immunofluorescence of monoclonal antibody to OTX1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of OTX1 expression in transfected 293T cell line by OTX1 monoclonal antibody (M01), clone 1F2.Lane 1: OTX1 transfected lysate(37.3 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged OTX1 is approximately 0.03 ng/ml as a capture antibody.
Western blot analysis of OTX1 over-expressed 293 cell line, cotransfected with OTX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with OTX1 monoclonal antibody (M01), clone 1F2 . GAPDH ( 36.1 kDa ) used as specificity and loading control.