Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of Osteocalcin (arrow) using rabbit polyclonal Osteocalcin Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BGLAP gene (Lane 2) (Origene Technologies).
Osteocalcin Antibody western blot of U251 cell line lysates (35 ug/lane). The Osteocalcin antibody detected the Osteocalcin protein (arrow).