Western blot analysis of extracts from untreated and dexamethasone-treated (50 nM, 16 h) HeLa cells, using Phospho-p57 Kip2 (Thr310) Antibody (upper) and p57 Kip2 Antibody #2557 (lower). Lysates were treated with lambda phosphatase NEB#P0753 (10,000 U/ml for 30 mins) to confirm the phospho-specificity of the phospho-antibody.
Confocal immunofluorescent analysis of HeLa cells, dexamethasone-treated (left) or phosphatase-treated (right), labeled with Phospho-p57 Kip2 (Thr310) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).