Western blot analysis of extracts from HeLa cells, untreated or treated with dexamethasone (50 nM, 16h) alone or with λ phosphatase, using p57 Kip2 Antibody #2557.
Western blot analysis, using a different p57 Kip2 mouse monoclonal antibody, of whole cell lysate from dexamethasone-treated (50 nM, 16h) HeLa cells (lane 1) and of p57 Kip2 immunoprecipitated from the same lysate using p57 Kip2 Antibody #2557 (lane 5). Lanes 2-4 represent controls for the immunoprecipitation experiment, as indicated.
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or dexamethasone-treated (right), using p57 Kip2 Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).