Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PEMT antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of lysates from CEM, rat L6 cell line and human liver tissue lysate(from left to right), using PEMT Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of anti-PEMT Antibody antibody in mouse kidney tissue lysates (35 ug/lane). PEMT(arrow) was detected using the purified antibody.