Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 Antibody (Center S320), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of lysates from A431, HeLa, U-937, BA/F3 cell line (from left to right), using PGK1 Antibody (S320). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.